Screening of Indigenous Rhizobacteria for Drought Stress Tolerance on Maize (Zea mays L) Seed Germination

Eko Hary Pudjiwati, Nur Indah Mansyur, Siti Zahara, Gina Husna

Abstract


Drought stress is still often a problem in maize cultivation, from germination to vegetative and generative growth. The germination process is hampered when there is drought, because imbibition is not perfect. Rhizobacteria have the potential to produce exopolysaccharides that can increase maize germination under drought stress conditions. This study aims to select indigenous rhizobacteria isolates that are able to provide better germination of maize seeds in drought conditions, using PEG 6000 10%. The number of selected rhizobacteria isolates was 20 isolates, and the study was repeated 2 times. Germination parameters observed were germination percentage (%), seedling height (cm), number of plumule leaves (strands), radicle length (cm), number of root hairs, wet weight of seedling (g), and dry weight of seedling (g). Observations were made when the sprouts were 7 days after planting (DAT). The results obtained 3 (three) rhizobacteria isolates, namely isolates B4(6), B4(8), and B5(19) which can increase seed viability and vigor under drought stress conditions. Isolate B4(8) increased germination percentage, seedling height, radicle length, number of root hairs and seedling wet weight. Isolate B5(19) can improve the percentage of germination, radicle length, number of root hairs, wet weight and dry weight of seedlings. Isolate B4(6) increased the number of root hairs and dry weight of seedlings.

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References


Efendi R, Azrai M. 2010. The response of maize genotypes to drought stress: the role of roots. Journal of Food Crops Agricultural Research. 29(1):1-10.

Marvasi M, Visscher PT, Casillas ML. 2010. Exopolymeric Substances (EPS) from Bacillus subtilis: polymers and genes encoding their synthesis. FEMS Microbiology Letters. 313 (1): 1-9.

Ozturk S, Aslim B. 2010. Modification of exopolysaccharide composition and production by three cyanobacterial isolates under salt stress. Environmental Science and Pollution Research. 17 (3): 595-602.

Santi LP, Ai Dariah, Goenadi DH. 2008. Increased stability of mineral soil aggregates by exopolysaccharide-producing bacteria. Plantation Tower 76 (2), 92-102.

Kapli H, Wahyudi AT, Husen E. 2017. Effect of growth promoting and drought tolerant rhizobacteria as well as abundance and activity of soil microbes on maize (Zea mays L.). Biospecies 10 (1): 25-36.

Efendi, R. 2009. Early Selection of Corn Genotype Tolerance to Drought. IPB. Bogor.

Santi, LP. 2011. The Role of Exopolysaccharide-Producing Bacteria in Sandy Texture Soil Aggregation (Dissertation). IPB. Bogor

Amartani, K. 2019. Response of corn (Zea mays L) seed germination under salt stress conditions. Agroscience 3 (1): 9-14.

Kurniati, S. 2018. Screening and Identification of Indole-3 Acetid Acid (IAA) Hormone-Producing Bacteria in Rice Roots (Oryza sativa) in Balang Village, Binamu District, Jeneponto Regency (Thesis). UIN Alauddin Makassar.

Anosike, M., Braide, E. E., Adeleye, W., (2018). Studies on Indole Acetic Acid (IAA) Production by Rhizobacteria and Growth promoting potentials. International Journal of Advanced Research in Biological Sciences. 5(2), 133-140.

Lakitan, B. 1996. Physiology of plant growth and development. PT Raja Grafindo Persada. Jakarta.

Sureshbabu, K., Amaresan, N., Kumar, K., 2016. Amazing multiple function properties of plant growth promoting rhizobacteria in the rhizosphere soil. Int. J. Curr. Microbiol.Appl. Sci 5 (2), 661–683.

Tefa, A. 2017. Viability and vigor test of rice seeds (Oryza sativa, L.) during storage at different moisture levels. Sandalwood Savanna. 2(3): 48-50.

Ilyas, S., Widajati, E. 2015. Techniques and Procedures for Testing Seed Quality. Bogor (ID): IPB Press.

Sutopo, L. 2004. Seed Technology. PT Raja Grafindo Persada. Jakarta.

Prawinata W., S. Harran and P. Tjondronegoro. 1992. Fundamentals of Plant Physiology. Faculty of Mathematics and Natural Sciences. Bogor Agricultural University.




DOI: https://doi.org/10.35334/iciksa.v0i0.56

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Jurnal J-Pen Borneo:Jurnal Ilmu Pertanian

Faculty of Agriculture, University Of Borneo Tarakan

Tarakan city, North Borneo Province, Indonesia