ANTAGONISTIC POTENCY ASSAY OF ANTIANTHRACNOSE PRODUCING BACTERIA AGAINST SEVERAL PHYTOPATHOGENIC FUNGUS
DOI:
https://doi.org/10.35334/iciksa.v0i0.190Abstract
Phytopathogenic fungus are the main obstacles in crop cultivation. The infection effect could lead to yield loss. The utilization of synthetic fungicides in controlling these fungus is harmful for the environment. Biological control using antagonistic bacteria offers an alternative choice to reduce that negative effect. The aim of this study was to test the antagonistic activity of antagonistic rhizobacteria and phyllobacteria isolates against several species of phytopathogenic fungus. Antifungal activity of four antagonistic bacteria isolates (UBCF_001, UBCF_013, UBCR_012, and UBCR_036) was assayed against three species of pathogenic fungus (Colletotrichum gloeosporioides, Fusarium oxysporum, and Sclerotium rolfsii). Antagonistic assay was conducted using paper diffusion method by applying 20 µl of culture onto whatmann filter paper. Bacterial inhibition against fungus was determined by comparing the growth rate of both treated and untreated fungus. Broad spectrum of antifungal activity was shown by rhizobacteria isolates (UBCR_012 and UBCR_036). Of those two isolates, UBCR_012 exhibited the highest antagonistic acitivity up to 51% against C. gloeosporioides isolated from dragon fruit. In contrast, phyllobacteria isolates (UBCF_001 and UBCF_013) showed specific inhibition against C. gloeosporioides only indicating its narrow spectrum of antifungal activity. Bacteria showing antagonistic activity against various pathogenic fungus offers promising prospect to be developed as biocontrol agents due to its high application value.References
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